November 17 SFASA Seminar
Berkeley
Genomics Seminar Co-Sponsored by the SF Bay Area Chapter of the American
Statistical Association
Title: Normalization
and Differential Expression in RNA-Seq
Speaker: Sandrine
Dudoit, Professor of Biostatistics and Statistics
Chair, Graduate Group in BiostatisticsUniversity of
California, Berkeley
http://www.stat.berkeley.edu/~sandrine
Location: 1011
Evans Hall
Time: Nov. 17th Thursday 4-5 pm
Abstract:
This talk concerns
statistical methods and software for the analysis of RNA abundance by
sequencing (RNA-Seq). We first present exploratory
data analysis (EDA) approaches for quality assessment/control (QA/QC) of RNA-Seq reads. Next, we propose within-lane normalization
methods to adjust for sample-specific gene-level effects such as length and
GC-content. We also provide between-lane normalization procedures to account
for distributional differences such as sequencing depth. Finally, we consider
the quantitation of (differential) gene expression levels using generalized
linear models (GLM). This work was motivated by a
collaboration with the Sherlock Lab on transcriptome
analysis in Saccharomyces. Our exploratory data analysis and normalization
methods are implemented in the open-source Bioconductor
R package EDASeq (http://www.bioconductor.org/ ).
P.S. Sandrine has already posted this seminar to be sponsored by us
on her website: http://www.stat.berkeley.edu/%7Esandrine/Teaching/PH292.F11/
Directions and maps:
1) UCB campus map from UCB Statistics department website (http://www.stat.berkeley.edu/58),
where you could see Evans Hall is highlighted in the map.
2) The driving direction to the campus is here: http://visitors.berkeley.edu/gethere/traveling.shtml
3) Parking map on UCB campus (visitor parking with symbol "P" ): http://pt.berkeley.edu/sites/pt.berkeley.edu/files/content/NewPadMap2011.pdf
4) Public transportation to UCB campus:
BART @Downtown Berkeley station (Exit the
Berkeley station at Center Street. Walk east on Center Street for 1 block to
the campus)
AC Transit: several AC bus lines that
stop at the campus at the intersection of Bancroft Way and Telegraph Avenue
such as lines: #1; #1R; #7; #49; #51B; #52; and F.
The SF Bay Area Chapter does not
schedule a seminar in December. However, this year, we have arranged to
co-sponsor a seminar with Stanford
Biostatistics on Thursday December 1 from 1:15 to 3:00 pm.
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